Kwilizwe le-elebhu ezenzekelayo, ukufumana izisombululo ezikwenza oko kukuthi ukusebenza kakuhle nokuchaneka kubalulekile. Ngokuqala kweplate ye-96--ngokugqibeleleyo, abaphandi kunye nezazinzulu zivule amandla enqanaba elitsha lokuzenzekelayo. Ezi plates zinika uluhlu lweempawu ezikhuthaza ukusebenza kohlalutyo, ukhuseleko esampulu, kunye nokudityaniswa kwetyhefu ngeenkqubo zerobhothi. Kwezi bhlog, siya kuqhuba kwiinkcukacha zeplate ye-96-pite ye-stuted kwaye uxoxe ngezinto eziluncedo kwizicelo ezahlukeneyo zelabhoratri.
Ukuphucula ukusebenza kakuhle:
Enye yeenzuzo ezibalaseleyo zepleyiti ezingama-96 ngokupheleleyo kukwanda kwandise ukusebenza kakuhle. Iipleyiti zenzelwe ukuba zihambelane nomgangatho we-ANDI ye-ANDI kwaye ihlawulwe kwiinkqubo ezenzekelayo, zilungiselela ukusetyenziswa kwendawo yelebhu exabisekileyo. Abaphandi ngoku banokwenza inani elikhulu kakhulu le-askey ngaxeshanye, ukuphucula imeko yokufaka, imveliso kunye nokusebenza ngendleko.
Ukuphucula ukusebenza nge-PCR:
Iprofayili ephantsi ye-96 - ipleyiti eyenziwe kakuhle ngokupheleleyo inceda ekunciphiseni indawo efileyo kwaye iphucule ukusebenza kwempumelelo ye-Polymerase Chain Reaction (PCR). I-PCR yindlela ephambili esetyenziselwa ukukhulisa i-DNA, kwaye naluphi na umahluko kwiqondo lobushushu ngaphakathi kweplate lunokubangela ukwandiswa okungahambelaniyo. Ukusetyenziswa kwezi plate kuqinisekisa ukudluliselwa kobushushu obungafaniyo, kunciphisa ukubakho kokungafani kobushushu, kwaye ekugqibeleni kunyuke ukuthembeka kunye nokuchaneka kweziphumo zePCR.
Ukuphathwa kwerobhothi ephuculweyo:
Ukudityaniswa okungagungqiyo kunye neenkqubo ezizenzekelayo, i-96 - iplate ye-stirt epheleleyo inikezelwa njengePhambili, enamaxesha amane ngakumbi. Eli nqaku libalulekileyo liqinisekisa ukuphatha kakuhle kwerobhothi kwaye linciphise umngcipheko weengozi kunye neempazamo ngexesha lokutshintshelwa kweplate. Izixhobo ezenzekelayo zihamba ngokuthembekileyo, iintlobo kunye neepeyinti, zikhokelela ekusebenzeni okumangalisayo kunye nokunciphisa ixesha lokuphumla.
Itywinwe ngokukhuselekileyo ngaphandle kokuphuma:
Ukukhuthaza imiphetho ejikeleze nganye i-quit iqhubela phambili itywina elikhuselekileyo ngokuchasene nokuphuma. Eli tywina libalulekile xa liphatha iisampulu ezibuthathaka ezifuna ulawulo oluchanekileyo lwevolumu kunye nendawo esingqongileyo. Abaphandi banokuphumla ngokulula abazi iisampulu zabo ezixabisekileyo zikhuselwe kungcoliseko kunye nokuphuma kwamanzi, ukuqinisekisa ukuba iziphumo zovavanyo eziqinisekileyo.
Udluliselo oluguqukayo lobushushu:
Ngokusebenzisa iindonga ezifanayo zekhephu, isiketi esipheleleyo se-96 ngokusisigxina sinika i-fed ephezulu kunye nokungaguquguquki kobushushu phakathi kwequla nganye. Oku kufana kubalulekile kwizikhuselo ezifuna ulawulo lobushushu oluchanekileyo, ezinje ngebhayisikile ye-thermy, ukuphendula kwe-enzymatic, kunye ne-proteli yeproto. Izakhono ezifanelekileyo zokudlulisela ubushushu obusebenzayo
IXESHA LOKUQALA: Jun-25-2023